Author: Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.
Title: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells Document date: 2009_10_7
ID: jgxbpy4j_13
Snippet: MS and MSMS data were analyzed, and peak lists generated using GPS Explorer 3.5 (Applied Biosystems). MS peaks were selected between 800 and 4000 Da and filtered with a signal to noise ratio greater than 15 and to exclude masses derived from trypsin autolysis (842. 51, 1006.48, 1045.56, 2211.1, 2283.18, and 2299.18) . MSMS peaks were selected on the basis of a signal to noise ratio greater than 10 over a mass range of 50 to 20 Da below the precur.....
Document: MS and MSMS data were analyzed, and peak lists generated using GPS Explorer 3.5 (Applied Biosystems). MS peaks were selected between 800 and 4000 Da and filtered with a signal to noise ratio greater than 15 and to exclude masses derived from trypsin autolysis (842. 51, 1006.48, 1045.56, 2211.1, 2283.18, and 2299.18) . MSMS peaks were selected on the basis of a signal to noise ratio greater than 10 over a mass range of 50 to 20 Da below the precursor mass. A peak density filter was used with no more than 30 peaks per 200 Da and a maximum number of peaks of 65.
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