Author: Hui-Yuen, Joyce; McAllister, Shane; Koganti, Siva; Hill, Erik; Bhaduri-McIntosh, Sumita
Title: Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines Document date: 2011_11_8
ID: kss857n1_10
Snippet: Step 4 is to be performed, you will need to incubate an additional flask of un-infected PBMC at 2 x 10 6 cells/ml as control. vi. single-stained with FITC conjugated isotype control antibody matched to CD58-FITC (at the same concentration as anti-CD58 antibody) vii. single-stained with PE-Cy5 conjugated isotype control antibody matched to CD19-PE-Cy5 (at the same concentration as anti-CD19 antibody) viii. triple-stained with all three isotype con.....
Document: Step 4 is to be performed, you will need to incubate an additional flask of un-infected PBMC at 2 x 10 6 cells/ml as control. vi. single-stained with FITC conjugated isotype control antibody matched to CD58-FITC (at the same concentration as anti-CD58 antibody) vii. single-stained with PE-Cy5 conjugated isotype control antibody matched to CD19-PE-Cy5 (at the same concentration as anti-CD19 antibody) viii. triple-stained with all three isotype control antibodies.
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