Beyond type 1 regulatory T cells: co-expression of LAG3 and CD49b in IL-10-producing T cell lineages

Weishan Huang; Sabrina Solouki; Chavez Carter; Song-Guo Zheng; Avery August

of Nb-infected mice express high levels of both LAG3 and CD49b (Fig. 2A&B) , and are 213 . CC-BY-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/359547 doi: bioRxiv preprint predominantly CD4 + T cells (Fig. 2C) . However interestingly, these IL-10-producing 214 LAG3 + CD49b + CD4 + T cells included both Foxp3 + and Foxp3 -CD4 + T cells subsets (Fig. 2C , last 215 plot; and Fig. 2D ). To further determine whether LAG3/CD49b co-expression is correlated with 216 IL-10 and/or Foxp3 expression in CD4 + T cells, we compared the percentage of LAG3/CD49b 217 double positive population in Foxp3 -IL-10 -, Foxp3 -IL-10 + , Foxp3 + IL-10and Foxp3 + IL-10 + CD4 + T 218 cells isolated from the lungs of Nb-infected mice. We found that regardless of expression of Foxp3, 219