Beyond type 1 regulatory T cells: co-expression of LAG3 and CD49b in IL-10-producing T cell lineages

Weishan Huang; Sabrina Solouki; Chavez Carter; Song-Guo Zheng; Avery August

proportions of IL-10-producing Foxp3 + CD4 + , Foxp3 -CD4and CD8 + subsets differed in the 241 various disease models (Fig. 3) . In contrast to composition of IL-10-producing LAG3 + CD49b + T 242 cells induced by Nb infection, in which Foxp3 -CD4 + subset is the majority (72% in Fig. 2B) , in 243 HDM-induced allergic asthma model, the largest subset of the IL-10-producing LAG3 + CD49b + T 244 cells in lungs are Foxp3 + CD4 + T cells (60%), followed by Foxp3 -CD4 + T cells (32%), while CD8 + 245 T cells are only around 1.4% (Fig. 3B) . In the SR-triggered farmer's lung disease model, Foxp3 + 246 CD4 + T cells are the largest majority of the IL-10-producing LAG3 + CD49b + T cell subset in the 247 lungs, however, there are similar percentages of Foxp3 -CD4 + and CD8 + T cells (16% each) (Fig. 248 3D) . Strikingly in the murine model of influenza infection, CD8 + T cells are the largest subset of 249 the IL-10-producing LAG3 + CD49b + T cells in the lungs (86%), followed by Foxp3 -CD4 + T cells, 250