Author: Mouzakis, Kathryn D.; Lang, Andrew L.; Vander Meulen, Kirk A.; Easterday, Preston D.; Butcher, Samuel E.
Title: HIV-1 frameshift efficiency is primarily determined by the stability of base pairs positioned at the mRNA entrance channel of the ribosome Document date: 2012_12_15
ID: ix8du1er_14
Snippet: Luminescence was measured using the dual-luciferase reporter assay (Promega) as previously described (57) . Readings were taken with a Veritas microplate luminometer equipped with dual-injectors (Turner Bio-systems) for 10 s after 25 ml of the respective substrate was injected into the reaction mixture (2-s lag time prior to measurement). Ratios of firefly/Renilla luminescence were calculated for each of the experimental and control translation r.....
Document: Luminescence was measured using the dual-luciferase reporter assay (Promega) as previously described (57) . Readings were taken with a Veritas microplate luminometer equipped with dual-injectors (Turner Bio-systems) for 10 s after 25 ml of the respective substrate was injected into the reaction mixture (2-s lag time prior to measurement). Ratios of firefly/Renilla luminescence were calculated for each of the experimental and control translation reactions. The frameshift efficiency was calculated by taking the ratio of the experimental/control luminescence (firefly/Renilla). Frameshift efficiencies were averaged and their standard deviations were propagated through to yield a standard error of the mean (SEM).
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