Reovirus Activates a Caspase-Independent Cell Death Pathway

Berger, Angela K.; Danthi, Pranav

To determine if infection with T1L also results in necroptosis, we assessed whether ATP is lost from T1L-infected cells. We found that~30% to 40% ATP was lost after T1L infection at 48 h (see Fig. S3A in the supplemental material). Thus, in comparison to T3D-infected cells (Fig. 4B) , ATP loss following infection with T1L is delayed. The loss of ATP in T1L-infected cells was able to be diminished by Nec-1 but not pan-caspase inhibitor treatment, suggesting that cell death following T1L infection also occurs via necroptosis. We also used AOEB staining to determine the kinetics and mechanisms of T1L-induced cell death. We found~10% and~30% cell death in cells infected with T1L at 48 and 72 h after infection, respectively (Fig. S3B ). Because cell death following T1L infection is sensitive to Nec-1 and is substantially lower than thẽ 60% cell death induced by T3D at 48 h after infection, our data indicate that T1L induces necroptosis in L929 cells with slower kinetics than T3D.