Selected article for: "adv multiplex pcr and PCR multiplex"
Author: Lee, Jong-Han; Chun, Jin-Kyong; Kim, Dong Soo; Park, Yongjung; Choi, Jong Rak; Kim, Hyon-Suk
Title: Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia
  • Document date: 2010_9_1
    • ID: jo29nx9b_11
    Snippet: Samples showing any positive results for AdV, IFV, PIFV, and RSV from the two multiplex PCR kits were performed with shell vial cultures by R-Mix ReadyCells (Diagnostic HYBRIDS, Inc., Athens, OH, USA) according to manufacturer's procedures. Firstly, the cryopreserved R-Mix cell reagents were heated in a 37ËšC heat block for 4 min, and rinsed with a rinse buffer and then remained for 4 min at room temperature. In the meantime, the re-feed medium a.....
    Document: Samples showing any positive results for AdV, IFV, PIFV, and RSV from the two multiplex PCR kits were performed with shell vial cultures by R-Mix ReadyCells (Diagnostic HYBRIDS, Inc., Athens, OH, USA) according to manufacturer's procedures. Firstly, the cryopreserved R-Mix cell reagents were heated in a 37˚C heat block for 4 min, and rinsed with a rinse buffer and then remained for 4 min at room temperature. In the meantime, the re-feed medium and 1.0 mL R-Mix ReadyCells were mixed. Then, 200 µL specimens were inoculated into R-Mix ReadyCells and centrifuged at 700×g for 60 min at room temperature. After overnight incubation at 35˚C in the incubator, cell monolayers of the shell vial were washed and fixed with acetone, and stained with respiratory virus fluorescent antibodies by D3 DFA (Diagnostic Hybrids, Inc., Athens, OH, USA). If virus-specific fluorescence was noted by

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