Selected article for: "model stem loop and stem loop"
Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
  • Document date: 2017_9_29
    • ID: k4gtl2o7_27
    Snippet: Formation of the RNA model stem-loop 5 to the slippage motif should act as a physical roadblock for the transcribing RT polymerase on the A-tract. We first identified the minimal number of nucleotides 5 of an A7 motif at which formation of the model stem-loop could stimulate slippage. Base C 5 adjacent to the motif was maintained in all sequences. Derivatives of the 'wtSL-A7' construct were made with 1, 2 or 3 nt insertions between the stem-loop .....
    Document: Formation of the RNA model stem-loop 5 to the slippage motif should act as a physical roadblock for the transcribing RT polymerase on the A-tract. We first identified the minimal number of nucleotides 5 of an A7 motif at which formation of the model stem-loop could stimulate slippage. Base C 5 adjacent to the motif was maintained in all sequences. Derivatives of the 'wtSL-A7' construct were made with 1, 2 or 3 nt insertions between the stem-loop and the A-tract motif (Supplementary Figure S2, panel A) .

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