Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_75
Snippet: To convert fluorescence brightness values to vesicle diameters, we computed a scaling factor that centered the mean of the vesicle brightness distribution of a high-curvature, sonicated vesicle sample to the average diameter of the same vesicles obtained from dynamic light scattering. This scaling factor was then used to scale the vesicle brightness distributions after protein exposure to distributions of vesicle diameter......
Document: To convert fluorescence brightness values to vesicle diameters, we computed a scaling factor that centered the mean of the vesicle brightness distribution of a high-curvature, sonicated vesicle sample to the average diameter of the same vesicles obtained from dynamic light scattering. This scaling factor was then used to scale the vesicle brightness distributions after protein exposure to distributions of vesicle diameter.
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