Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_8
Snippet: For the experiments included in that publication, the controls to distinguish whether indels in its product DNA derived from the initial DNA-dependent RNA polymerase step, or subsequently from product cDNA reverse transcriptase slippage, revealed no reverse transcriptase slippage. Follow-up work tested potential nascent RNA stemloop structure stimulation of slippage at runs of A shorter than 9, the minimal needed for efficient slippage at such mo.....
Document: For the experiments included in that publication, the controls to distinguish whether indels in its product DNA derived from the initial DNA-dependent RNA polymerase step, or subsequently from product cDNA reverse transcriptase slippage, revealed no reverse transcriptase slippage. Follow-up work tested potential nascent RNA stemloop structure stimulation of slippage at runs of A shorter than 9, the minimal needed for efficient slippage at such motifs. In this unpublished work, a significant proportion of the reverse transcriptase product of one 75 nt chemically synthesized RNA template with an inverted repeat with potential to form the 'model' stem-loop 5 adjacent to 8 A's, had an extra T. This control experiment prompted the present investigation of RNA template stemloop structure-mediated reverse transcriptase realignment.
Search related documents:
Co phrase search for related documents- reverse transcriptase and RNA polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- reverse transcriptase and RNA template: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- reverse transcriptase and significant proportion: 1, 2, 3, 4
- reverse transcriptase and transcriptase product: 1, 2, 3, 4
- reverse transcriptase and transcriptase slippage: 1, 2, 3, 4, 5
- reverse transcriptase and unpublished work: 1
- reverse transcriptase realignment and RNA polymerase: 1, 2
- reverse transcriptase realignment and RNA template: 1
- reverse transcriptase realignment and transcriptase slippage: 1
- reverse transcriptase slippage and RNA polymerase: 1, 2, 3, 4
- reverse transcriptase slippage and RNA template: 1
- reverse transcriptase slippage and transcriptase slippage: 1, 2, 3, 4, 5
- RNA polymerase and significant proportion: 1, 2, 3
- RNA polymerase and transcriptase slippage: 1, 2, 3, 4
- RNA template and transcriptase product: 1
- RNA template and transcriptase slippage: 1
- stemloop structure and template stemloop: 1
Co phrase search for related documents, hyperlinks ordered by date