Author: Gao, Shuibo; Li, Enyao; Gao, Haixia
Title: Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway Document date: 2019_8_21
ID: jwrv3e6j_15
Snippet: Firstly, HT22 cells were exposed to 10 mM morphine for 24 h. The OX1R, c-fos, p/t-ERK and p/t-PKC expression were tested. Data in Figure 1 (A) showed that 10 mM morphine exposure dramatically elevated the OX1R protein level in HT22 cells (p Ë‚ 0.01). The c-fos protein level in HT22 cells was also increased after 10 mM morphine exposure (Figure 1(B) , p Ë‚ 0.01). Moreover, 10 mM morphine exposure notably enhanced the p/t-ERK and p/t-PKC expression.....
Document: Firstly, HT22 cells were exposed to 10 mM morphine for 24 h. The OX1R, c-fos, p/t-ERK and p/t-PKC expression were tested. Data in Figure 1 (A) showed that 10 mM morphine exposure dramatically elevated the OX1R protein level in HT22 cells (p Ë‚ 0.01). The c-fos protein level in HT22 cells was also increased after 10 mM morphine exposure (Figure 1(B) , p Ë‚ 0.01). Moreover, 10 mM morphine exposure notably enhanced the p/t-ERK and p/t-PKC expression rates in HT22 cells (Figure 1 (C), p Ë‚ 0.05 or p Ë‚ 0.01). These outcomes illustrated that morphine could elevate OX1R and c-fos expression and activate ERK and PKC pathways in HT22 cells.
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