Selected article for: "Bio Rad imaging system and nonfat milk"
Author: Hernandez, Nicholas; Melki, Isabelle; Jing, Huie; Habib, Tanwir; Huang, Susie S.Y.; Danielson, Jeffrey; Kula, Tomasz; Drutman, Scott; Belkaya, Serkan; Rattina, Vimel; Lorenzo-Diaz, Lazaro; Boulai, Anais; Rose, Yoann; Kitabayashi, Naoki; Rodero, Mathieu P.; Dumaine, Cecile; Blanche, Stéphane; Lebras, Marie-Noëlle; Leung, Man Chun; Mathew, Lisa Sara; Boisson, Bertrand; Zhang, Shen-Ying; Boisson-Dupuis, Stephanie; Giliani, Silvia; Chaussabel, Damien; Notarangelo, Luigi D.; Elledge, Stephen J.; Ciancanelli, Michael J.; Abel, Laurent; Zhang, Qian; Marr, Nico; Crow, Yanick J.; Su, Helen C.; Casanova, Jean-Laurent
Title: Life-threatening influenza pneumonitis in a child with inherited IRF9 deficiency
  • Document date: 2018_10_1
    • ID: jqv0lyfx_40
    Snippet: Knockdown efficiency was determined by immunoblotting. 30 μg of total protein loaded per lane were separated on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen) and then transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). After blocking with 5% nonfat dry milk (Bio-Rad Laboratories) in PBS containing 0.1% Tween 20 (Sigma-Aldrich), membranes were blotted with the IRF9 antibody (ISGF-3γ p48.....
    Document: Knockdown efficiency was determined by immunoblotting. 30 μg of total protein loaded per lane were separated on 4-12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen) and then transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). After blocking with 5% nonfat dry milk (Bio-Rad Laboratories) in PBS containing 0.1% Tween 20 (Sigma-Aldrich), membranes were blotted with the IRF9 antibody (ISGF-3γ p48 [H10]), MAVS (clone 3993; Cell Signaling Technologies), or HSP90 (clone 68; BD Biosciences), followed by incubation with appropriate HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) and SuperSignal West Pico Chemiluminescent substrate or SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific). Signal was detected by ChemiDocTM Touch Imaging System (Bio-Rad Laboratories).

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