In Situ Tolerance within the Central Nervous System as a Mechanism for Preventing Autoimmunity

Brabb, Thea; von Dassow, Peter; Ordonez, Nadia; Schnabel, Bryan; Duke, Blythe; Goverman, Joan

Mice. MBP TCR1 transgenic mice (B10.PL-H2 u -H2-T18 a [73NS]/Sn-TgN [TCR ␣ ]/BlJg and B10.PL-H2 u -H2-T18 a [73NS]/Sn-TgN [TCR ␤ ]/C14Jg [11] ) and MBP TCR2 transgenic mice (12) were backcrossed onto B10.PL (73 NS)/Sn for at least 11 generations. B10.PL (73 NS)/Sn, C57Bl/6J, and lymphocytic choriomeningitis virus (LCMV) TCR transgenic mice (B6, D2-TgN (TCR LCMV) 327Sdz [15] ) were obtained from The Jackson Laboratory. Ovalbumin (Ova) TCR transgenic mice (C57BL/6-TgN [TCR Ova] 1100Mjb [16] ) and E ␣ 52-68:I-A b (TEa) TCR transgenic mice (17) were provided by Dr. Michael Bevan (University of Washington, Seattle, WA) and Dr. Alexander Rudensky (University of Washington, Seattle, WA), respectively. DO11.10 TCR transgenic mice were provided by Dr. Anne O'Garra (DNAX Research Institute, Palo Alto, CA). Mice were maintained in a specific pathogen-free facility. The Animal Care Committee of the University of Washington approved all procedures used in this report.