Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus Document date: 2014_6_16
ID: k785hl1r_15
Snippet: The purified VLPs were analyzed by electron microcopy as previously described [21] . Briefly, 200-mesh carboncoated copper grids were floated on drops of chimeric VLPs for 10 min, then washed with water three times. Next, 2% phosphotungstic acid (PH 7.0) was applied for 1 min for negative staining. After the girds were air-dried, the samples were examined by transmission electron microscopy (JEM-100CX-II; JEOL, Japan) at a magnification of 20,000.....
Document: The purified VLPs were analyzed by electron microcopy as previously described [21] . Briefly, 200-mesh carboncoated copper grids were floated on drops of chimeric VLPs for 10 min, then washed with water three times. Next, 2% phosphotungstic acid (PH 7.0) was applied for 1 min for negative staining. After the girds were air-dried, the samples were examined by transmission electron microscopy (JEM-100CX-II; JEOL, Japan) at a magnification of 20,000×.
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