Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo

Zhou, Ping; Zhai, Shanli; Zhou, Xiang; Lin, Ping; Jiang, Tengfei; Hu, Xueying; Jiang, Yunbo; Wu, Bin; Zhang, Qingde; Xu, Xuewen; Li, Jin-ping; Liu, Bang

Seven genes (CCL2, SLC39A14, ATP6V1B2, C3, DDIT3, GLRX2 and TNF) were selected for Q-PCR assay to validate the changes in gene expression observed by microarray analysis. CCL2 was the main upregulated chemokine gene in this study (Table 4) . Two upregulated genes, SLC39A14 and ATP6V1B2, were involved in intracellular zinc homeostasis and endosome acidification, respectively. The downregulated C3 gene is the core member of the complement system which seemed to be inhibited, according to our study ( Table 4 ). The Q-PCR gene list also contained two DE genes (DDIT3, GLRX2) which were not referred to in the discussion, and TNF, an important cytokine gene, which was not differentially expressed in Tongcheng PAMs in response to HP-PRRSV infection. The changes of these genes, detected by microarray analysis, was in agreement with the Q-PCR validation (Figure 4) .