Selected article for: "HCV detection and quantitation detection"
Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing
  • Document date: 2012_11_30
    • ID: jiy4cp4n_21
    Snippet: RT-PCR was introduced to amplify RNA targets. In this method, cDNA is first produced from RNA by reverse transcription and then the cDNA is amplified by PCR. A thermostable DNA polymerase derived from Thermus thermophilus could function efficiently as both an RT and a DNA polymerase [14] . Commercially kits are available for detection of HCV RNA and for quantitation of HIV-1 and HCV RNA in clinical specimens (Roche Diagnostics, Indianapolis, IND).....
    Document: RT-PCR was introduced to amplify RNA targets. In this method, cDNA is first produced from RNA by reverse transcription and then the cDNA is amplified by PCR. A thermostable DNA polymerase derived from Thermus thermophilus could function efficiently as both an RT and a DNA polymerase [14] . Commercially kits are available for detection of HCV RNA and for quantitation of HIV-1 and HCV RNA in clinical specimens (Roche Diagnostics, Indianapolis, IND).

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