Selected article for: "proofreading exonuclease and RNA polymerase"
Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
  • Document date: 2017_9_29
    • ID: k4gtl2o7_4
    Snippet: Despite these studies of transcription slippage, and several studies of reverse transcriptase fidelity including (34) (35) (36) (37) , significant issues concerning RT mediated indel formation merit investigation. The use of RT as a lab reagent is one of the reasons why their slippage propensity is of interest (38) . However, RTs do not contain 3 exonuclease proofreading activity and their templates are prone to form structures at the ambient tem.....
    Document: Despite these studies of transcription slippage, and several studies of reverse transcriptase fidelity including (34) (35) (36) (37) , significant issues concerning RT mediated indel formation merit investigation. The use of RT as a lab reagent is one of the reasons why their slippage propensity is of interest (38) . However, RTs do not contain 3 exonuclease proofreading activity and their templates are prone to form structures at the ambient temperature at which these enzymes act. Better reagent polymerases have been developed from thermophilic DNA-dependent DNA polymerases by adapting their catalytic activity to function with RNA templates (39) (40) (41) , or derived from existing RT polymerase by genetic engineering (42) . Though the derived enzymes have the beneficial quality of lower base mis-incorporation due to higher accuracy for substrate selection (39) , their indel fidelity remains to be explored.

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