Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_53
Snippet: The results here show that a cassette from Drosophila retrotransposon Dme1 chrX 2630566 containing an AU6 motif, exhibits strong slippage with sensitivity to relative dNTP concentration conditions. In addition, a cassette with a Maize retrotransposon sequence that has conserved potential for template stem-loop structure formation 5 to a motif A4C3, showed marginal slippage-mediated addition of T. Given the widespread occurrence and importance of .....
Document: The results here show that a cassette from Drosophila retrotransposon Dme1 chrX 2630566 containing an AU6 motif, exhibits strong slippage with sensitivity to relative dNTP concentration conditions. In addition, a cassette with a Maize retrotransposon sequence that has conserved potential for template stem-loop structure formation 5 to a motif A4C3, showed marginal slippage-mediated addition of T. Given the widespread occurrence and importance of retrotransposons, these results highlight the need for systematic studies to reveal the extent of their functional utilization of RT slippage.
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