Author: Zhang, Yongli; Zhang, Hangjie; Ma, Wenqiang; Liu, Kefang; Zhao, Min; Zhao, Yingze; Lu, Xuancheng; Zhang, Fuping; Li, Xiangdong; Gao, George F.; Liu, William J.
Title: Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1(-/-) Mice Document date: 2018_10_17
ID: km6fnf5u_12
Snippet: Immunogenic T-cell epitope plays a significant role in cellular immunity against pathogens 23 . Thus, the detection of ZIKV-specific T cells in immunoprivileged organs is a critical methodology to understand T-cell responses against the natural ZIKV infection. Meanwhile, T-cell response detection is an excellent tool to investigate the efficacy of the viral vaccine. Here, we show a comprehensive protocol to visualize the experiments, which includ.....
Document: Immunogenic T-cell epitope plays a significant role in cellular immunity against pathogens 23 . Thus, the detection of ZIKV-specific T cells in immunoprivileged organs is a critical methodology to understand T-cell responses against the natural ZIKV infection. Meanwhile, T-cell response detection is an excellent tool to investigate the efficacy of the viral vaccine. Here, we show a comprehensive protocol to visualize the experiments, which include the isolation of lymphocytes from the spleen, brain, and testes of ZIKV-infected mice, the preparation of the immunodominant epitope E 294-302 tetramer, and the recognition of ZIKV-specific CD8 + T cells in immunoprivileged organs of ZIKV-infected mice.
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