Author: Hernes, S. S.; Quarsten, H.; Hagen, E.; Lyngroth, A. L.; Pripp, A. H.; Bjorvatn, B.; Bakke, P. S.
Title: Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs Document date: 2010_9_18
ID: ivu632j2_24
Snippet: The other real-time PCR was performed using 5 μl of (c) DNA in a 20-μl reaction mixture consisting of 5 mM MgCl 2 , 750 units uracil-DNA glycosylase (Eurogentec S. A., Seraing, Belgium), LightCycler FastStart DNA Master Mix (Roche cat. no. 12239272001), 0.5-1.0 μM of the primers and 0.2-0.4 μM of each of the probes. All primers and probes are listed in Table 2 . The PCR cycling conditions were initiated with 2 min at 42°C, followed by 10 min.....
Document: The other real-time PCR was performed using 5 μl of (c) DNA in a 20-μl reaction mixture consisting of 5 mM MgCl 2 , 750 units uracil-DNA glycosylase (Eurogentec S. A., Seraing, Belgium), LightCycler FastStart DNA Master Mix (Roche cat. no. 12239272001), 0.5-1.0 μM of the primers and 0.2-0.4 μM of each of the probes. All primers and probes are listed in Table 2 . The PCR cycling conditions were initiated with 2 min at 42°C, followed by 10 min at 95°C and 47 cycles of 15 s at 95°C, 30 s at 60°C and 20 s at 72°C.
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