Selected article for: "sucrose pad and velocity gradient"
Title: Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells
  • Document date: 1992_5_1
    • ID: j3vo4zkj_10
    Snippet: In preliminary experiments, centrifugal force x time was varied in sucrose velocity gradients to resolve different organelles . Approximately 0.2 ml was layered over a 0.3-1 .5 M buffered sucrose gradient with a 0.4-ml 2 M sucrose pad at the bottom and centrifuged 100,000 g (32 .5 k rpm) for 1 h (except where noted) in a SW55Ti rotor. Gradients were collected from the bottom, 200 A1 per fraction . A 25,000-g, 1-h gradient was found to separate Go.....
    Document: In preliminary experiments, centrifugal force x time was varied in sucrose velocity gradients to resolve different organelles . Approximately 0.2 ml was layered over a 0.3-1 .5 M buffered sucrose gradient with a 0.4-ml 2 M sucrose pad at the bottom and centrifuged 100,000 g (32 .5 k rpm) for 1 h (except where noted) in a SW55Ti rotor. Gradients were collected from the bottom, 200 A1 per fraction . A 25,000-g, 1-h gradient was found to separate Golgi fractions from immature secretory granules in agreement with , but did not allow the smallest sulfate-containing vesicles to enter the gradient sufficiently. A 100,000-g, 1-h sucrose velocity gradient resolved these vesicles from free label at the top of the gradient .

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