Selected article for: "elispot assay and IFN number"

Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
  • Document date: 2014_6_16
  • ID: k785hl1r_34
    Snippet: Cellular immune responses were evaluated through detection of the number of splenocytes secreting IFN-γ or IL-4 using the ELISPOT assay. Single-cell suspensions of splenocytes prepared from the spleens of thrice-vaccinated mice were stimulated by inactivated H120 virus. The results showed that chimeric VLPs elicited higher levels of IL-4 production than inactivated H120 viruses (p < 0.01; Fig. 4A ), while both induced comparable IFN-γ product.....
    Document: Cellular immune responses were evaluated through detection of the number of splenocytes secreting IFN-γ or IL-4 using the ELISPOT assay. Single-cell suspensions of splenocytes prepared from the spleens of thrice-vaccinated mice were stimulated by inactivated H120 virus. The results showed that chimeric VLPs elicited higher levels of IL-4 production than inactivated H120 viruses (p < 0.01; Fig. 4A ), while both induced comparable IFN-γ production ( Fig. 4B) , suggesting that the chimeric VLPs could elicit both Th1 and Th2 type cellular immune responses in mice.

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