Author: Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min
Title: Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head Document date: 2016_9_13
ID: kjvy2c2k_11
Snippet: Surgical procedure and perfusion. The surgical procedure was performed as previously reported (26) . In brief, the animals were anesthetized with intravenous pentobarbital, and a lateral approach was made to expose the greater trochanter under aseptic conditions. A drill with an diameter of 1 mm was inserted at the flare of the greater trochanter and into the necrotic area of the femoral head, assisted by a C-arm X-ray machine. The necrotic tissu.....
Document: Surgical procedure and perfusion. The surgical procedure was performed as previously reported (26) . In brief, the animals were anesthetized with intravenous pentobarbital, and a lateral approach was made to expose the greater trochanter under aseptic conditions. A drill with an diameter of 1 mm was inserted at the flare of the greater trochanter and into the necrotic area of the femoral head, assisted by a C-arm X-ray machine. The necrotic tissue was then removed completely, and the cell-hydrogel composites were transplanted into the necrotic area through the bone tunnel made by drill for Groups III and IV. The bone tunnel was sealed with an absorbable collagen sponge plug, and the wound was closed in layers. Following the operation, animals received gentamicin (80 MU/day; Shanghai No.1 Biochemical & Pharmaceutical Co., Ltd., Shanghai, China) intramuscularly as prophylaxis for 3 days, and all animals were free to move.
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