Author: Noh, Heeju; Shoemaker, Jason E; Gunawan, Rudiyanto
Title: Network perturbation analysis of gene transcriptional profiles reveals protein targets and mechanism of action of drugs and influenza A viral infection Document date: 2018_4_6
ID: j80hnhpb_10
Snippet: For influenza A infection analysis, we obtained the raw microarray data of four influenza studies (27) (28) (29) (30) from GEO database (accession numbers: GSE40844, GSE37571, GSE33142 and GSE28166). The raw data were background-corrected and normalized using normexp and quantile methods in limma package of Bioconductor. The log 2 FCs and their adjusted P-values were again calculated by limma. The probes were mapped to the corresponding gene symb.....
Document: For influenza A infection analysis, we obtained the raw microarray data of four influenza studies (27) (28) (29) (30) from GEO database (accession numbers: GSE40844, GSE37571, GSE33142 and GSE28166). The raw data were background-corrected and normalized using normexp and quantile methods in limma package of Bioconductor. The log 2 FCs and their adjusted P-values were again calculated by limma. The probes were mapped to the corresponding gene symbols using hgug4112a.db package (Entrez Gene database as of 27 September 2015). Like before, for genes with multiple probe sets, we chose the log 2 FC value corresponding to the probe set with the smallest average adjusted P-value.
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