Author: Lin, Zhaoru; Gilbert, Robert J. C.; Brierley, Ian
Title: Spacer-length dependence of programmed -1 or -2 ribosomal frameshifting on a U(6)A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting Document date: 2012_6_28
ID: kjet3e50_11
Snippet: Frameshift reporter plasmids were linearized with Nco I or Bam H1 and capped run-off transcripts generated using SP6 RNA polymerase as described (12) . Messenger RNAs were recovered by a single extraction with phenol/ chloroform (1:1 vol/vol) followed by ethanol precipitation. Remaining unincorporated nucleotides were removed by gel filtration through a NucAway spin column (Ambion)......
Document: Frameshift reporter plasmids were linearized with Nco I or Bam H1 and capped run-off transcripts generated using SP6 RNA polymerase as described (12) . Messenger RNAs were recovered by a single extraction with phenol/ chloroform (1:1 vol/vol) followed by ethanol precipitation. Remaining unincorporated nucleotides were removed by gel filtration through a NucAway spin column (Ambion).
Search related documents:
Co phrase search for related documents- ethanol precipitation and vol vol: 1
Co phrase search for related documents, hyperlinks ordered by date