2015 ACVIM Forum Research Abstract Program

The administration of metronidazole led to significant changes in the fecal microbiome as evaluated by qPCR. Interestingly, metronidazole led to a decrease in bacterial groups that constitute major abundant groups in the GI tract of healthy dogs and are thought to be important for maintaining intestinal health (Faecalibacterium, Blautia, and Ruminococcaceae). While most of the changes were reversed 14 days after the end of antibiotic administration, some of the evaluated bacterial groups remained significantly altered up to 4 weeks after administration. In conclusion, metronidazole has a profound effect on the fecal microbiome and leads to a more variable microbiome over time. Further studies are needed to evaluate the functional changes in the microbiome that occur due to metronidazole, to better understand the mechanisms of antibiotics in gastrointestinal disease. Gastrokines (GKNs) are bioactive substances secreted by selected gastric cells. Evidence supports functional roles for GKNs in gastric homeostasis, immune responses and tumour suppression. Down-regulation has been reported in Helicobacter pylori associated gastritis and other inflammatory gastrointestinal conditions. The aim of this study was to develop a quantitative polymerase chain reaction (qPCR) assay for utilization to evaluate GKN gene expression in Helicobacter spp positive dogs before and after treatment.