2015 ACVIM Forum Research Abstract Program

Sensitivity was 2.0 mg/dL. Observed to expected (O/E) ratios for 3 serial dilutions of urine samples averaged 110.00%, ranging from 80.73 to 124.75%. Spiking recoveries in 3 replicates of 3 urine samples of varying specific gravities averaged 132.66%, ranging from 118.87 to 143.60%. Coefficients of variations for intra-assay variability ranged from 3.45 to 5.19%, while coefficients of variation for inter-assay variability ranged from 4.61 to 7.27% in three urine samples of varying specific gravities. The reference range for urine 5-HIAA:Creatinine in 20 clinically healthy dogs was 0.01 -0.12. Urinary 5-HIAA:Creatinine values in dogs with gastroenteropathies were significantly greater than in healthy dogs (median 5-HIAA:Creatinine 0.04 vs 0.08, healthy dogs vs dogs with enteropathy, P = 0.0034, Mann Whitney test), and showed a significant linear relationship (P = 0.0002, R 2 = 0.369) between urinary 5-HIAA:Creatinine and CIBDAI, a scoring system for severity of canine gastrointestinal disease.