2015 ACVIM Forum Research Abstract Program

Consecutive feline urine samples that were yellow and had <5 RBC and WBC per hpf were used. Samples were pooled to a final volume ≥ 7 mL (n = 30 pools). Feline blood (1:20 dilution of blood in urine) was added to an aliquot of each pool. Serial dilutions (1:2) were performed with the remainder of each pool. The undiluted pool and the 1:20, 1:40, 1:80, 1:160, 1:320, 1:1280 and 1:5280 dilutions were evaluated in a blinded fashion. Urine sample color was recorded (light yellow, dark yellow, light pink, dark pink, or red). Pools were centrifuged and supernatants collected and stored at À20C. Protein (colorimetric assay) and creatinine concentrations were measured via automated clinical chemistry analyzer. A signed rank test was used to compare UPCR between the undiluted samples and each color. Also, results for the light and dark yellow were averaged and called "yellow" and for the light and dark pink and red were averaged and called "colored". Data were compared between undiluted, "yellow" and "colored" using a repeated measures ANOVA on ranks; post hoc comparisons were made via the Tukey test. A Chi-square was used to compare categorizations (normal, borderline or high) between colors. Significance was set at the P < 0.05 level. (1747) 0 0 3 0 UPCR were significantly increased (P < 0.001) in "colored" and "yellow" samples compared to the undiluted samples and in all individual colors compared to undiluted. Proteinuria categorizations differed significantly (P < 0.001) between undiluted and all colors except light yellow (see table) . Proteinuria categorizations differed significantly between undiluted and "colored" but not between undiluted and "yellow".