Selected article for: "agarose gel and Sanger sequencing"
Title: 2015 ACVIM Forum Research Abstract Program
  • Document date: 2015_5_27
    • ID: 3pnuj5ru_265
    Snippet: The objective of this study was to identify the feline AIP gene, identify SNPs within the feline AIP gene and compare any SNPs with reported human AIP SNPs. Stored pituitary tissue from an acromegalic cat was used to create and amplify feline AIP cDNA using feline specific AIP primers. Stored EDTA blood from 10 acromegalic cats (diagnosis of insulin resistant diabetes mellitus, serum IGF-1 > 1000 ng/ml and pituitary mass >4 mm identified using co.....
    Document: The objective of this study was to identify the feline AIP gene, identify SNPs within the feline AIP gene and compare any SNPs with reported human AIP SNPs. Stored pituitary tissue from an acromegalic cat was used to create and amplify feline AIP cDNA using feline specific AIP primers. Stored EDTA blood from 10 acromegalic cats (diagnosis of insulin resistant diabetes mellitus, serum IGF-1 > 1000 ng/ml and pituitary mass >4 mm identified using contrast-enhanced pituitary computed tomography or at necropsy) and 10 control cats (no history of diabetes mellitus and greater than 15 years of age) were selected, DNA extracted and genotyped using PCR, agarose gel electrophoresis and Sanger sequencing.

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