Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_169
Snippet: FOSCC biopsies were graded and immunohistochemistry used to quantify relative expression of Bcl11b, EGFR, Ki67, and VEGF-D. Chi-square tests determined if Bcl11b expression correlated with expression of other receptors and growth factors. Western blots on FOSCC cell lines (SCCF1, SCCF2, SCCF3) were employed to verify expression of Bcl11b, EGFR, Src, ERK, and VEGF-D. Western blot on FOSCC cell lines determined phosphorylation of Bcl11b and the eff.....
Document: FOSCC biopsies were graded and immunohistochemistry used to quantify relative expression of Bcl11b, EGFR, Ki67, and VEGF-D. Chi-square tests determined if Bcl11b expression correlated with expression of other receptors and growth factors. Western blots on FOSCC cell lines (SCCF1, SCCF2, SCCF3) were employed to verify expression of Bcl11b, EGFR, Src, ERK, and VEGF-D. Western blot on FOSCC cell lines determined phosphorylation of Bcl11b and the effect of a src inhibitor, dasatinib, on this activation. An MTT assay was applied on FOSCC cell lines following treatment with dasatinib. ELISA quantified VEGF production by FOSCC cells with and without dasatinib treatment.
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