2016 ACVIM Forum Research Abstract Program

The purpose of this study was to evaluate the cross-reactivity of feline insulin with the Iso-Insulin ELISA using purified feline insulin standards. Five purified feline insulin standards with known concentrations ranging from 9.13 ng/L to 702 ng/L that are used as calibrators for the commercial feline insulin ELISA (Mercodia AB, Uppsala, Sweden) were used. The ELISAs were performed in duplicate according to the manufacturer's recommendations. The mean absorbance values of the feline insulin ELISA calibrators were below the mean absorbance value of the lowest Iso-Insulin calibrator (0.087) except for the first calibrator, which had a slightly higher mean absorbance of 0.099. The slightly higher absorbance for the first calibrator was likely the result of a minor technical issue with this very sensitive ELISA since all calibrators with higher insulin concentrations consistently had absorbances at or below the lowest Iso-insulin calibrator.