2015 ACVIM Forum Research Abstract Program

Fresh serum samples from healthy dogs (n = 10) were collected after physical examination and were used to perform a serum chemistry profile and measure serum cobalamin and canine trypsin-like immunoreactivity (cTLI) concentrations. These healthy dogs had serum cobalamin and cTLI concentrations within the respective reference intervals of 251-908 ng/L and 5.7-45.2 lg/L. Diseased groups were both hypocobalaminemic (serum cobalamin concentration < 150 ng/L; low B 12 ) and were prospectively enrolled. A group of 15 hypocobalaminemic dogs without EPI and a group of 13 hypocobalaminemic dogs with EPI (cTLI concentration of ≤ 2.5 lg/L; EPI) were enrolled. A previously validated gas chromatography/mass spectrometry method was used to quantify serum glycine, glutamic acid, methionine, tyrosine, tryptophan, and lysine concentrations of the dogs enrolled. A Kruskal-Wallis test and a Dunn's post-test were performed to compare the concentrations of the six amino acids amongst healthy dogs, and hypocobalaminemic dogs with or without EPI.