2015 ACVIM Forum Research Abstract Program

Cobalamin-deficient Shar-Peis had higher CNV-Eastern assay copy numbers (median [range]: 5.0 [2.0-7.0]) and lower CNV_23.759 assay copy numbers (2.1 [1.0-8.6]) than normocobalaminemic Shar-Peis (2.5 [1.7-7.1] and 5.3 [2.4-11.6], respectively; both: P < 0.0001). Receiver-operating characteristic curves were used to determine the optimal copy number that distinguished cobalamin-deficient from normocobalaminemic Shar-Peis. High copy numbers (≥4.5) in the CNV-Eastern assay was detected in 12 (60%) of the cobalamin-deficient Shar-Peis, and cobalamin deficiency was associated with a high CNV-Eastern copy number (OR: 25.5 [CI: 4.7-137.4]; P < 0.0001). The CNV_23.759 assay revealed low copy numbers (≤2.5) in 12 (60%) of the cobalamindeficient Shar-Peis, and cobalamin deficiency was associated with a low CNV_23.759 copy number (OR: 52.5 [CI: 5.9-464.6]; P < 0.0001). A high CNV-Eastern copy number, low CNV_23.759 copy number, and high CNV-Eastern or low CNV_23.759 copy number (OR: 81.7 [CI: 9.0-741.4]; P < 0.0001) distinguished cobalamin-deficient Shar-Peis from normo-cobalaminemic Shar-Peis with a sensitivity of 60, 60, and 70%, respectively, and a specificity of 94, 97, and 97%, respectively.