2015 ACVIM Forum Research Abstract Program

In conclusion, measurement of 3-BrY using electron impact ionization gas chromatography/mass spectrometry is sensitive, linear, accurate, precise, and reproducible for use with fecal sample from dogs. Studies assessing the clinical utility of measurement of 3-BrY in fecal samples from dogs are warranted and in progress. Spirocerca lupi is a nematode that infects the caudal esophagus in dogs, which serve as the final host. S. lupi induces the formation of fibro-inflammatory, non-neoplastic nodules in the esophagus of which 25% transform into neoplastic lesions. Eosinophilic infiltration is rarely found within nodules and only early and transient eosinophilia occurs experimentally. 3-Bromotyrosine (3-BrY) is a biomarker of eosinophilic activation. We hypothesized that serum 3-BrY concentrations may be increased in dogs infected with S. lupi and that 3-BrY may be useful to discriminate between benign and malignant nodules. The objective of this study were to compare serum 3-BrY concentrations in dogs infected with S. lupi with those in healthy dogs and to compared serum 3-BrY concentrations in dogs with benign and those with malignant nodules.