Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_557
Snippet: The supernatant of isolates made from a dog and from a foal that died from these diseases was highly cytotoxic for an equine ovarian (EO) cell line. Partial genome sequencing of the dog isolate revealed three novel putative toxin genes related to the poreforming Leukocidin/HemolysinSuperfamily. These were designated netE, netF and netG. Both netE and netF were located on one large conjugative plasmid, and netG was located with the cpe enterotoxin.....
Document: The supernatant of isolates made from a dog and from a foal that died from these diseases was highly cytotoxic for an equine ovarian (EO) cell line. Partial genome sequencing of the dog isolate revealed three novel putative toxin genes related to the poreforming Leukocidin/HemolysinSuperfamily. These were designated netE, netF and netG. Both netE and netF were located on one large conjugative plasmid, and netG was located with the cpe enterotoxin gene on a second large conjugative plasmid. netE and netF were found in all EO-cytotoxic isolates, as was cpe, but netG was less consistently present.
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