Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_400
Snippet: In this crossover study, units of blood were collected from 8 healthy dogs. In half of the units, leukocytes and platelets were removed via leukoreduction. Units of pRBCs were then created via centrifugation and stored for 10 or 21 days. Initial baseline plasma samples were collected after processing and before storage (Day 0). Additional samples were collected following storage for 10 or 21 days, both after removal from refrigeration and again a.....
Document: In this crossover study, units of blood were collected from 8 healthy dogs. In half of the units, leukocytes and platelets were removed via leukoreduction. Units of pRBCs were then created via centrifugation and stored for 10 or 21 days. Initial baseline plasma samples were collected after processing and before storage (Day 0). Additional samples were collected following storage for 10 or 21 days, both after removal from refrigeration and again after 5 hours at room temperature (to simulate transfusion conditions). Concentrations (ng/mL) of arachidonic acid (AA), prostaglandin E 2 (PGE 2 ), prostaglandin D 2 (PGD 2 ), prostaglandin F 2a (PGF 2a ), thromboxane B 2 (TXB 2 , stable metabolite of TXA 2 ), 6-keto-prostaglandin F 1a (6-keto-PGF 1a , stable metabolite of prostacyclin) and leukotriene B 4 (LTB 4 ) were quantified by liquid chromatography-mass spectrometry.
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