2016 ACVIM Forum Research Abstract Program

Staff and student owned dogs were recruited at a Veterinary Teaching Hospital in a B. burgdorferi non-endemic area. Inclusion criteria stipulated that dogs were apparently healthy, weighed at least 10 kg, and had not been administered a Leptospira spp. vaccine within the past year. The dogs were randomized to be administered one of four commercially available Leptospira spp. vaccines that all contained serovars Canicola, Gryppotyphosa, Icterohemorrhagiae, and Pomona. Blood was collected prior to vaccine administration on Week 0 and Week 3 and then again on Weeks 4, 8, and 12. Leptospira spp. microagglutination titers (MAT) were determined using a commercially available service. After confirming the maximal Leptospira spp. titers occurred on Week 4, an aliquot of this sera was shipped to Antech Diagnostics for analysis of B. burgdorferi antibodies against OspA, OspC, and OspF with the Accuplex 4 BioCD system.