2016 ACVIM Forum Research Abstract Program

The 2 dogs with anemia, thrombocytosis and neutropenia on the day prednisolone was initiated were clinically normal, AP PCR negative, and were normal in all other CBC evaluations, suggesting these results were spurious. The one dog in Group 2 that was positive for AP DNA on Week 25, was also positive on Week 24 prior to prednisolone administration. None of the other samples assessed by CBC and AP PCR assay after initiation of prednisolone administration showed evidence for activation of AP infection, suggesting that this protocol is unlikely to adversely affect dogs with chronic AP infection in the field. Whether other immune suppressive protocols would activate chronic AP infection remains to be proven. The only dogs that became AP seronegative by Week 48 were administered doxycycline, suggesting treatment may aid in the elimination of chronic AP infection, even after prednisolone administration. The objective of this study was to determine the proportion of feline calicivirus (FCV) field isolates that would be neutralized in vitro by sera from cats that were routinely vaccinated with a product containing two caliciviruses. Serum from cats hyperinoculated against 2 feline caliciviruses (FCV) has been shown to cross neutralize a greater proportion (90.2%) of 61 FCV field isolates from the United States when compared to serum from cats hyperinoculated with 1 FCV isolate (23.0%) At 8 and 11 weeks of age, each of the 44 kittens was administered an experimental vaccine containing modified-live panleukopenia, modified-live feline herpesvirus 1, and non-adjuvanted inactivated calicivirus strains FCV-255 and FCV-DD1. Serum was collected from each kitten 7 and 14 days after the second dose of vaccine and pooled by day.