2015 ACVIM Forum Research Abstract Program

Twenty metabolites were identified that were significantly (p < 0.05) different between remission and control groups. The majority of amino acids (alanine, asparagines, glutamine, homoserine, leucine, methionine, phenylalanine, proline, serine, tyrosine and valine) that were significantly changed were decreased by 25 to 66%, and were negatively correlated with glucose parameters (casual blood glucose, fasting blood glucose, 2 h reading during GTT, and return to baseline during GTT). The exception was glycine, which had a more than two-fold increase, and was positively correlated with glucose parameters. Three fatty acids were identified as being significantly different (after adjustment for body condition score). Hexadecenoic (palmitoleic) acid and octadecanoic (stearic) acid were decreased with moderate negative correlations to glucose parameters, and 1-mono hexadecanoyl glycerol (1palmitoylglycerol) was increased, but had little correlation with glucose parameters. While the majority of cats in remission were able to maintain normal fasting blood glucose concentration, their fasting glucose concentrations were 30% higher than control cats when analysed by mass spectrometry. The sugar alcohol xylitol was decreased in remission cats and correlated negatively with all glucose parameters. The organic acid a-ketoglutaric acid and the inorganic acid phosphoric acid were increased and decreased respectively, but were not significantly correlated with glucose parameters. Urea was also increased approximately 70% in remission cats compared to control cats, even after removal of 6 cats with IRIS stage ≥ 2 renal disease, and was positively correlated with both 2 hour glucose and time to return to baseline.