2015 ACVIM Forum Research Abstract Program

Compared with control group, the fold-changes of the both target genes were significantly low in PDA (0.50 AE 0.17 for SER-CA2a and 0.41 AE 0.21 for PLN) as well as in PS (0.47 AE 0.20 for SERCA2a and 0.35 AE 0.10 for PLN). In paired comparison between pre-and post-operation, their expression levels significantly increased one week after surgical correction in both PDA (0.74 AE 0.22 for SERCA2a and 0.68 AE 0.24 for PLN) and PS (0.74 AE 0.18 for SERCA2a and 0.65 AE 0.20 for PLN). Meanwhile, plasma levels of NT-proBNP between before and after surgery were not different in both PDA (before surgery, 761.0 AE 579.2 pmol/L; one week after surgery, 679.9 AE 437.2 pmol/L) and PS (before surgery, 867.2 AE 560.2 pmol/L; one week after surgery, 863.4 AE 523.1 pmol/L). Additionally, the expression levels of SER-CA2a and PLN were significantly correlated to hemodynamic indices, such as fractional shortening and LA/Ao ratio in PDA, and peak pressure gradient in PS (P < 0.05). Furthermore, receiveroperating characteristic analysis showed high values of the area under the curve in SERCA2a (0.95 AE 0.04 in PDA; 0.95 AE 0.04 in PS) and PLN (1.00 AE 0.0 in PDA; 0.98 AE 0.03 in PS).