2015 ACVIM Forum Research Abstract Program

Consecutive canine urine samples submitted for urinalysis that were yellow and had no glucose on dipstick evaluation were used. Sample supernatants were pooled based on USG to obtain a final volume of ≥ 3 mL. Pools were divided into ranges by USG: USG of 1.005-1.009, 1.010-1.014, 1.015-1.019, 1.020-1.024, 1.025-1.029, 1.030-1.034, 1.035-1.039, and 1.040-1.045 and named range A, B, C, D, E, F, G, H and I, respectively, with five pools in each range. A solution of 0.1 g/mL glucose was added to an aliquot of each pool to obtain a final concentration of 2,400 mg/dL. Serial dilutions were performed with the remainder of each pool to obtain glucose concentrations of 1,200, 600, 300, 150 and 50 mg/dL. The USG was measured in the undiluted samples and all spiked samples with a handheld refractometer (Reichert TS 400). For statistical comparisons, the change in USG from the undiluted sample was calculated and divided by the final glucose concentration (in g/dL) in the sample. A repeated measures ANOVA on ranks was used to compare the change in USG per g/dL glucose between the USG ranges. Post hoc comparisons were made with the Tukey test. Median values are reported. Significance was set at the p < 0.05 level.