2015 ACVIM Forum Research Abstract Program

An indirect enzyme-linked immunosorbent assay (ELISA) was developed to semi-quantitatively evaluate the ability of two monoclonal antibodies (MAbs) that target bovine TF surface antigens (MAbs 1.15, 1.17) to recognize feline TF. ELISA positivity was defined as two standard deviations above the mean of a negative control. Qualitative assessment was performed using dot blot analysis. Co-culture of porcine intestinal epithelial cells (IPEC-J2) with MAb 1.15, 1.17-treated TF was performed to evaluate the effect of these surface antigens on intestinal epithelial cytotoxicity. A bovine TF and 1 Pentatrichomonas hominis (PH) isolate were used as positive and negative controls, respectively. Assays were performed in a minimum of triplicate. Data were analyzed using Systat (p < 0.05).