2016 ACVIM Forum Research Abstract Program

A total of six (three male; three female) one-year-old purpose bred cats were included in the IACUC approved study. The cats were previously maintained as unvaccinated controls in a FHV-1 study at five months of age and none of the cats had clinical signs of FHV-1 when enrolled in the current study. On Week 0, blood, serum, urine were collected for biomarker assays and a wedge kidney biopsy for histopathological evaluation and alpha-enolase immunohistochemistry was obtained. After sample collection on Week 0 and again on Weeks 2, 4, 6, 8, 10, 12, and 14, all cats were administered a commercially available FVRCP vaccine shown previously to induce anti-CRFK and anti-enolase antibodies and samples were collected for biomarker assays. After sample collection on Week 16, similarly sized renal biopsies were made, avoiding the previous biopsy sites. Haematoxylin and eosin stained sections were provided to two board-certified pathologists that were masked to the timing of the biopsies. Anti-CRFK and antienolase antibodies levels in serum were determined by ELISAs.