2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

A crossmatch was performed using both the tube and gel method for 10 cats (total of 31 crossmatches). For 9 recipient cats (24 crossmatches), there was agreement between results of the tube and gel methods, with the exception of 1 cat having a 2+ and 3+ major incompatibility to the same donor with the tube and gel methods, respectively, likely a result of the subjectivity of grading. Differing results observed for 1 cat, with a negative tube but 4+ mixed field agglutination gel result for autocontrol and major crossmatch tests with 3 donors, were attributed to marked rouleaux. Microscopic evaluation and saline replacement during the tube crossmatch supported an interpretation of rouleaux rather than hemagglutination.