Author: Yeung, Siu-Wai; Lee, Thomas Ming-Hung; Cai, Hong; Hsing, I-Ming
Title: A DNA biochip for on-the-spot multiplexed pathogen identification Document date: 2006_9_25
ID: 0sg0hv9w_3
Snippet: In a previous study, our group demonstrated a proof-ofconcept experiment that both DNA amplification by the PCR and sequence-specific electrochemical amplicon detection could be done in a single microchamber (8) , in contrast to the commonly used device which involved multi-chambers with complex microfluidic control elements (9) . This microdevice had an 8 mL reaction chamber etched in a silicon substrate with a thin-film heater and temperature s.....
Document: In a previous study, our group demonstrated a proof-ofconcept experiment that both DNA amplification by the PCR and sequence-specific electrochemical amplicon detection could be done in a single microchamber (8) , in contrast to the commonly used device which involved multi-chambers with complex microfluidic control elements (9) . This microdevice had an 8 mL reaction chamber etched in a silicon substrate with a thin-film heater and temperature sensor patterned on top for rapid thermal cycling. An oligonucleotide capture probe-modified detection electrode was placed on a glass substrate used to seal the microchamber. To develop this prototype device into practical use, sample preparation functionality as well as the ability to perform multiplexed analysis would need to be addressed.
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