Author: Horiuchi, Sho; Saito, Yuichi; Matsui, Atsuka; Takahashi, Nobumasa; Ikeya, Tomohiko; Hoshi, Eishin; Shimizu, Yoshihiko; Yasuda, Masanori
Title: A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations Document date: 2020_1_14
ID: 4sltubqk_2
Snippet: Previously, direct DNA sequencing was the standard method for detecting genetic mutations (10) ; however, at present, several alternative methods for mutation testing have been developed (11, 12) . For example, the Therascreen EGFR PCR kit ® (Qiagen, Inc.) is a commercial quantitative (q)PCR kit and has been widely adopted for clinical practice; however, this method is time-consuming and possesses certain procedural complexities, for example, re.....
Document: Previously, direct DNA sequencing was the standard method for detecting genetic mutations (10) ; however, at present, several alternative methods for mutation testing have been developed (11, 12) . For example, the Therascreen EGFR PCR kit ® (Qiagen, Inc.) is a commercial quantitative (q)PCR kit and has been widely adopted for clinical practice; however, this method is time-consuming and possesses certain procedural complexities, for example, requiring several temperature changes during DNA amplification (11) . Next-generation sequencing has improved the efficiency of oncogene testing by high-throughput sequencing, which can detect dozen of mutations at the same time, but the high-cost of this technique limits its clinical usage (13, 14) . Therefore, detecting oncogenic mutations using a simple, easy and highly reproducible method remains a challenge.
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