2015 ACVIM Forum Research Abstract Program

We conclude that the commercially available 5-HIAA ELISA described here is sufficiently sensitive, linear, accurate, precise, and reproducible for use in diluted canine urine samples. Further, we conclude that there is a correlation between 5-HIAA excreted in urine and severity of gastroenteropathic disease in canines as assessed by the CIBDAI scoring system. 3-Bromotyrosine (3-BrY) has been used as a marker for eosinophil mediated diseases in humans. In a recent study, serum 3-BrY concentrations were shown to be increased in dogs with chronic enteropathy. However, fecal samples may be preferable to serum samples for evaluating the activation of eosinophils within the gastrointestinal tract. A method for the measurement of 3-BrY concentrations in fecal samples from dogs has not yet been established. Therefore, the aims of this study were to (1) develop and analytically validate a method for measuring 3-BrY concentrations in fecal samples from dogs using electron impact ionization gas chromatography/mass spectrometry and (2) establish a reference interval for fecal 3-BrY concentrations in healthy control dogs.