Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_603
Snippet: In total, 55 dogs were included in this study (28 dogs with and 27 dogs without tick or flea infestation) regardless of their health status. Whole blood and serum samples were collected for complete blood count and blood chemistry profile. Twenty tick and nine flea pool samples were collected from 28 dogs with tick or flea infestation and morphologically identified. DNA samples were extracted from whole blood, ticks and fleas. Polymerase chain re.....
Document: In total, 55 dogs were included in this study (28 dogs with and 27 dogs without tick or flea infestation) regardless of their health status. Whole blood and serum samples were collected for complete blood count and blood chemistry profile. Twenty tick and nine flea pool samples were collected from 28 dogs with tick or flea infestation and morphologically identified. DNA samples were extracted from whole blood, ticks and fleas. Polymerase chain reactions (PCR) targeting to each pathogen were performed. DNA sequencing was performed on all positive DNA fragments for genetic information. Association of vector-borne pathogen infections and age, sex, presence of anemia, thrombocytopenia, evidence of tick or flea infestation, use of tick or flea control products, and allowing of free roaming were assessed using Fisher's exact test.
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