Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_608
Snippet: The lowest reliable level of detection for C. cayetanensis DNA was 1fM of DNA/reaction and the assay was linear over a range of four dilutions with an r 2 value of 0.926 and a reaction efficiency of 102%. The melting curve showed that the Tm values for the C. cayetanensis oligonucleotide ranged between 81.1°C and 81.8°C. Amplification of G. duodenalis, C. parvum, and T. gondii DNA was not observed. Optimal annealing temperature was 57°C. Enter.....
Document: The lowest reliable level of detection for C. cayetanensis DNA was 1fM of DNA/reaction and the assay was linear over a range of four dilutions with an r 2 value of 0.926 and a reaction efficiency of 102%. The melting curve showed that the Tm values for the C. cayetanensis oligonucleotide ranged between 81.1°C and 81.8°C. Amplification of G. duodenalis, C. parvum, and T. gondii DNA was not observed. Optimal annealing temperature was 57°C. Enteric parasites were detected in 8 of 60 cats (13.3%) and included Cryptosporidium spp. (6.7%), Giardia spp. (3.3%) and I. felis (3.3%). DNA of C. cayetanensis was not amplified from any sample. For those samples with adequate DNA for sequencing, the 18S rRNA assay typed only to Cryptosporidium spp. for four isolates and two isolates typed as C. felis. The bgiardin and the glutamate dehydrogenase genes typed one Giardia spp. as Assemblage F.
Search related documents:
Co phrase search for related documents- 18S rRNA assay and dna reaction: 1
- Cryptosporidium spp type and Giardia spp type: 1, 2, 3
- dehydrogenase gene and dna reaction: 1, 2
Co phrase search for related documents, hyperlinks ordered by date