Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_227
Snippet: Methods: In this study, TCC cell lines highly expressing Hedgehog pathway mediators were treated with the inhibitors cyclopamine and GANT61. Down-regulation of the pathway was confirmed by real time RT-PCR for Patched 1 (PTCH1), GLI1 and GLI2. Cell growth was assessed at 48 and 96 hours after inhibitor treatment. Cell viability was assessed through the Alamar Blue assay. Apoptosis was assessed through Annexin V staining......
Document: Methods: In this study, TCC cell lines highly expressing Hedgehog pathway mediators were treated with the inhibitors cyclopamine and GANT61. Down-regulation of the pathway was confirmed by real time RT-PCR for Patched 1 (PTCH1), GLI1 and GLI2. Cell growth was assessed at 48 and 96 hours after inhibitor treatment. Cell viability was assessed through the Alamar Blue assay. Apoptosis was assessed through Annexin V staining.
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